Proteotyping as alternate typing method to differentiate Campylobacter coli clades
نویسندگان
چکیده
منابع مشابه
Molecular typing of Campylobacter jejuni and Campylobacter coli.
Shi et al. (7) presented data on the development and application of a PCR-based restriction fragment length polymorphism analysis of a 9.6-kb portion of the lipopolysaccharide gene (LG) cluster of Campylobacter jejuni and Campylobacter coli. This proposed molecular subtyping system is novel and has the potential of being directly applicable to epidemiology, prevalence, and other studies. I agre...
متن کاملDevelopment of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli.
A bacteriophage typing system for Campylobacter jejuni and Campylobacter coli was developed with phages isolated from poultry feces. Data for phage selection were generated from a set of isolates of C. jejuni and C. coli from humans in Illinois. Selection of 14 phages from the 47 phages available was assisted by determination of the Sneath-Jaccard similarity coefficients and subsequent unweight...
متن کاملPhenotypic and genotypic methods for typing Campylobacter jejuni and Campylobacter coli in poultry.
Human campylobacteriosis, an infection caused by the bacterium Campylobacter, is a major issue in the United States food system, especially for poultry products. According to the Center for Disease Control, campylobacterosis is estimated to affect over 2.4 million people annually. Campylobacter jejuni and Campylobacter coli are 2 species responsible for the majority of campylobacterosis infecti...
متن کاملSequence typing and comparison of population biology of Campylobacter coli and Campylobacter jejuni.
A multilocus sequence typing (MLST) scheme that uses the same loci as a previously described system for Campylobacter jejuni was developed for Campylobacter coli. The C. coli-specific primers were validated with 53 isolates from humans, chickens, and pigs, together with 15 Penner serotype reference isolates. The nucleotide sequence of the flaA short variable region (SVR) was determined for each...
متن کاملTyping of heat-stable and heat-labile antigens of Campylobacter jejuni and Campylobacter coli by coagglutination.
A coagglutination system has been devised for typing heat-stable and heat-labile antigens of Campylobacter jejuni and C. coli. The use of protein A-positive Staphylococcus aureus cells carrying Campylobacter sp. serotype antibody and the treatment of Campylobacter sp. cells with DNase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as s...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-40842-w